Thermo Fisher Scientific ATP Synthase beta Monoclonal Antibody (3D5AB1)
ATP Synthase β 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체(3D5AB1). Western blot, ICC/IF 등 다양한 응용에 적합하며, 사람 및 설치류 등 여러 종에 반응. 1 mg/mL 농도의 액상 정제 항체로 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 61 publications |
| Immunohistochemistry (IHC) | – | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 15 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunoprecipitation (IP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, C. elegans, Human, Mouse, Non-human primate, Rat |
| Published Species | C. elegans, Fruit fly, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D5AB1 |
| Immunogen | Human Heart Mitochondria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_221512 |
Product Specific Information
This antibody was produced in vitro using hybridomas grown in serum-free medium and purified by biochemical fractionation. Near homogeneity was confirmed by SDS-PAGE.
The epitope recognized by the anti-ATP synthase beta 3D5AB1 antibody is located in the region containing the active site of the beta-subunit, centered approximately at amino acid residue 83. The complete amino acid sequence of the epitope is not known.
Reference:
Chi SL, Wahl ML, Kenan DJ et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 67:4716–4724.
Target Information
ATP synthase is a highly conserved transmembrane enzyme found in plants, fungi, bacteria, and animals. It catalyzes the reversible conversion of ADP and phosphate to ATP, driven by proton flux across the membrane during electron transport.
The enzyme consists of two major regions:
- F1 ATP-ase (soluble): composed of α, β, γ, δ, and ε subunits
- F0 ATP-ase (membrane embedded): composed of a, b, and c subunits
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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