Thermo Fisher Scientific ATP Synthase beta Monoclonal Antibody (3D5AB1)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 61 publications 61 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 15 publications 15 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 7 publications 7 publications

Product Specifications

Species Reactivity

Bovine, C. elegans, Human, Mouse, Non-human primate, Rat

Published species

C. elegans, Fruit fly, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D5AB1

Immunogen

Human Heart Mitochondria. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP Synthase beta,uniProtId:P06576-1,ncbiNodeId:9606,antigenRange:1-529,antigenLength:529,antigenImageFileName:A-21351_ATP_Synthase_beta_P06576-1_House_mouse.svg,antigenImageFileNamePDP:A-21351_ATP_Synthase_beta_P06576-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_221512

Product Specific Information

The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Near homogeneity was judged by SDS-PAGE.

The epitope recognized by the anti-ATP synthase beta 3D5AB1 is in the region containing the active site of the beta-subunit and is centered approximately at amino acid residue 83. The complete amino acid sequence of the epitope is not known. See Chi SL, Wahl ML, Kenan DJ et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 67:4716-4724.

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.