Thermo Fisher Scientific TOP2A Monoclonal Antibody (OTI1D5), TrueMAB
TOP2A 단백질을 인식하는 Mouse monoclonal antibody. Western Blot과 IHC(P)에서 검증됨. Human 시료에 반응. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. DNA 합성과 세포분열 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1100–1531 of human TOP2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Topo II alpha plays important roles in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker of late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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