Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), PerCP-eFluor 710, eBioscience
인간 IL-10을 인식하는 JES3-9D7 단클론 항체로, PerCP-eFluor 710 형광체로 결합되어 있습니다. 유세포분석용으로 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 광민감성이 있으므로 암조건에서 보관하며, 4°C에서 냉장 보관합니다.
- 카탈로그번호
- 46-7108-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.03 µg)/test | View 9 publications |
| ELISA | - | View 1 publication |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES3-9D7 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573832 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
Description:
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested for intracellular staining and flow cytometry using stimulated human peripheral blood cells with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and BestProtocols® Protocol A.
Recommended usage: 5 µL (0.03 µg) per test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test (empirical).
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with a 488 nm blue laser.
Recommended filter: 710/50 bandpass (or 695/40 alternative).
Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation; protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal signal loss.
Excitation/Emission:
488 nm / 710 nm (Blue Laser)
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes and some lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages.
It promotes B cell survival, proliferation, and antibody production, and inhibits NF-kappa B activity.
IL-10 is involved in JAK-STAT signaling regulation.
Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Associated diseases include Graft-Versus-Host Disease and HIV Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.