Thermo Fisher Scientific GAD65, 67 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from aa region 520-585 of mouse GAD65 conjugated to blue carrier protein was used as the antigen. The peptide is identical in GAD67 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAD65/GAD67,uniProtId:P48318-1,ncbiNodeId:10090,antigenRange:520-585,antigenLength:593,antigenImageFileName:OSG00106W-100UL_GAD65GAD67_P48318-1_Rabbit.svg,antigenImageFileNamePDP:OSG00106W-100UL_GAD65GAD67_P48318-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:GAD65/GAD67,uniProtId:P48320-1,ncbiNodeId:10090,antigenRange:520-585,antigenLength:585,antigenImageFileName:OSG00106W-100UL_GAD65GAD67_P48320-1_Rabbit.svg,antigenImageFileNamePDP:OSG00106W-100UL_GAD65GAD67_P48320-1_Rabbit_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The antigen is homologous in rat and human.

Specificity of this antibody: GAD65, 67.

Target Information

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.