Thermo Fisher Scientific GAD65, 67 Polyclonal Antibody
GAD65 및 GAD67 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 동결건조 형태로 제공되며, 장기 보관 시 -20°C에서 안정적으로 유지. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:1,000
Miscellaneous PubMed (Misc)
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from aa region 520–585 of mouse GAD65 conjugated to blue carrier protein. The peptide is identical in GAD67. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in rat and human.
- Specificity: GAD65, 67.
Target Information
GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins responsible for catalyzing the rate-limiting step in GABA (gamma-aminobutyric acid) production from L-glutamic acid.
Both proteins are found in the brain:
- GAD-65: Localized to synaptic vesicle membranes in nerve terminals.
- GAD-67: Distributed throughout the cell and responsible for basal GABA synthesis.
During increased neurotransmission demand, GAD-65 transiently activates to assist GABA production. Loss of GAD-65 impairs GABA neurotransmission, while loss of GAD-67 is lethal.
Two isoforms exist for GAD-67 due to alternative splicing: the predominant GAD-67 and minor GAD-25 forms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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