Thermo Fisher Scientific Superoxide Dismutase 1 (SOD1) (Antioxidant Enzyme) Monoclonal Antibody (SOD1, 4330)
SOD1 단백질을 인식하는 마우스 모노클로날 항체로, Western blot과 IHC(P) 등 다양한 응용 가능. Human SOD1에 반응하며 단백질 정제는 Protein A/G 방식. PBS 완충액에 보관되며, 항산화 효소 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SOD1, 4330 |
| Immunogen | Recombinant fragment (around aa14–148) of human SOD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein that acts as a homodimer to convert harmful superoxide radicals into molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis (ALS). Rare transcript variants have also been reported.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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