Thermo Fisher Scientific Superoxide Dismutase 1 (SOD1) (Antioxidant Enzyme) Monoclonal Antibody (SOD1, 4330)
SOD1 단백질을 인식하는 mouse monoclonal 항체로, Western blot 및 IHC(P) 등 다양한 응용에 사용 가능. Human 시료에 반응하며, Protein A/G로 정제된 1 mg/mL 액상 형태. PBS(pH 7.4) buffer에 보존제 없이 제공되며, -20°C 이하에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SOD1, 4330 |
| Immunogen | Recombinant fragment (around aa14–148) of human SOD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed tissues): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein acting as a homodimer to convert harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis (ALS). Rare transcript variants have also been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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