Thermo Fisher Scientific Phospho-HSP27 (Ser78) Polyclonal Antibody
Phospho-HSP27 (Ser78) 폴리클로날 항체로 인간, 마우스, 랫트 반응성. WB, IHC, ICC, Flow, ELISA 등 다양한 응용 가능. 단백질 A로 정제된 1 mg/mL 액상 형태. 스트레스 반응 및 단백질 폴딩 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Ser78 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
In response to adverse environmental changes, cells increase expression of heat shock proteins (HSPs).
HSPB1 (heat shock protein beta-1, also known as HSP27) is a small heat shock protein functioning as a molecular chaperone, maintaining denatured proteins in a folding-competent state.
It plays roles in stress resistance and actin organization, and regulates biological processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in HSPB1 can lead to Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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