Thermo Fisher Scientific HIST1H2BA Monoclonal Antibody (OTI1C9), TrueMAB
HIST1H2BA 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1C9). Western blot과 IHC(Paraffin)에서 검증됨. 고순도 친화 크로마토그래피 정제. PBS/BSA/glycerol 완충액에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–127 of human HIST1H2BA produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells. It replaces classical histone H2B prior to the nucleosome-to-protamine transition and likely acts as a nucleosome dissociating factor, creating a more dynamic chromatin structure. Also expressed maternally and present in the female pronucleus, suggesting a role in protamine replacement by nucleosomes at fertilization. Found in fat cells, though its specific function and post-translational modifications in such cells remain unclear.
Histones are core components of nucleosomes, wrapping and compacting DNA into chromatin and regulating DNA accessibility for transcription, repair, replication, and stability. DNA accessibility is modulated by post-translational modifications of histones and nucleosome remodeling, collectively known as the histone code.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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