Thermo Fisher Scientific NARF Polyclonal Antibody
Thermo Fisher Scientific의 NARF Polyclonal Antibody는 인간 및 생쥐 시료에 반응하는 Rabbit IgG 항체로, Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 고농도 액상 형태로 장기 보관 시 -20°C 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–239 of Human NARF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548018 |
Product Specific Information
- Recommended positive controls: A549, H1299, HCT116, MCF-7, mouse muscle
- Predicted reactivity: Mouse (92%), Rat (93%), Bovine (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Several proteins are prenylated and methylated at their carboxyl-terminal ends. Prenylation, initially considered important only for membrane attachment, also plays a role in protein-protein interactions.
The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A undergoes farnesylation and carboxymethylation at the cysteine residue of a CaaX motif, which is later removed during maturation to lamin A.
The protein encoded by the NARF gene binds to the prenylated prelamin A carboxyl-terminal tail domain and may form part of a prelamin A endoprotease complex. It localizes to the nucleus and partially colocalizes with the nuclear lamina. NARF shares limited sequence similarity with iron-only bacterial hydrogenases.
Alternatively spliced transcript variants encoding different isoforms have been identified, including one generated by RNA editing.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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