Thermo Fisher Scientific SIRT1 Monoclonal Antibody (7B7)
SIRT1 단백질을 인식하는 Thermo Fisher Scientific의 Mouse monoclonal antibody (Clone 7B7). Western blot, IHC, ICC, ELISA에 적합하며, 고순도의 affinity chromatography로 정제된 액상 항체. Human SIRT1 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7B7 |
| Immunogen | SIRT1 (AAH12499, 456 a.a. – approximately 555 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein Sequence:
NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD
VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKSTarget Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through deacetylation of histones and can promote alterations in histone and DNA methylation, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and mediates the inhibitory effect on skeletal myoblast differentiation under low nutrient conditions, involving AMPK and NAMPT pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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