Thermo Fisher Scientific SIRT1 Monoclonal Antibody (7B7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

7B7

Immunogen

SIRT1 (AAH12499, 456 a.a. approximately 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIRT1,uniProtId:Q96EB6-1,ncbiNodeId:9606,antigenRange:456-555,antigenLength:747,antigenImageFileName:H00023411-M01_SIRT1_Q96EB6-1_House_mouse.svg,antigenImageFileNamePDP:H00023411-M01_SIRT1_Q96EB6-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKS

Target Information

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5`-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.