Thermo Fisher Scientific SPRED1 Polyclonal Antibody
인간 SPRED1 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot 및 Immunocytochemistry에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SPRED1. Recombinant protein control fragment (Product # RP-105211) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791669 |
Product Specific Information
Immunogen sequence:
GGSGLSSVTV FKVPHQEENG CADFFIRGER LRDKMVVLEC MLKKDLIYNK VTP
Target Information
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins.
Spred1 acts by suppressing the phosphorylation and activation of Raf.
The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis.
Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC.
Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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