Thermo Fisher Scientific C1q Monoclonal Antibody (3R9/2)
인간 C1q 단백질을 인식하는 Mouse monoclonal antibody (Clone 3R9/2). Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. Protein A로 정제된 액상 항체로, 4°C 단기 및 -20°C 장기 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | Assay-dependent | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3R9/2 |
| Immunogen | Globular head domain of C1q, purified from human plasma |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | BBS, pH 8.4 |
| Contains | < 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2067385 |
Product Specific Information
- Suggested positive control for immunohistochemical applications: kidney tissue from patients with streptococcal glomerulonephritis.
Strong staining observed in glomeruli and occasionally in peritubular capillary (potentially clinically significant). - Mouse anti-human C1q antibody, clone 004-43.X (3R9/2), recognizes human complement component 1q (C1q), an approximately 156 kDa secreted protein.
- C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system.
Target Information
C1q is a subcomponent of complement C1. It associates with complement components C1r and C1s, which are essential for the proteolytic complement cascade.
Deficiency of C1q has been linked to lupus erythematosus and glomerulonephritis.
C1q consists of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region near the N terminus and a C-terminal globular region.
The A-, B-, and C-chains are arranged in the order A–C–B on chromosome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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