Thermo Fisher Scientific HLA-DQA1 Recombinant Rabbit Monoclonal Antibody (JU17-34)
HLA-DQA1 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공. Western blot, IHC, IP 등 다양한 응용 가능. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용 가능.
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Thermo Fisher Scientific HLA-DQA1 Recombinant Rabbit Monoclonal Antibody (JU17-34)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU17-34 |
| Immunogen | Synthetic peptide within Human HLA-DQA1 (aa 205–254) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810109 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production candidates.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
HLA-DQA1 binds peptides derived from antigens that access the endocytic route of antigen-presenting cells (APCs) and presents them on the cell surface for recognition by CD4 T-cells. The peptide binding cleft accommodates peptides of 10–30 residues.
Peptides presented by MHC class II molecules are generated by degradation of proteins processed by lysosomal proteases. Exogenous antigens endocytosed by APCs are presented via MHC II molecules, the exogenous antigen presentation pathway.
Endogenous peptides can also be presented via autophagy, where autophagosomes fuse with MHC class II loading compartments.
In addition to APCs, epithelial cells of the gastrointestinal tract express MHC class II molecules and CD74, functioning as APCs.
For antigen presentation, three MHC class II heterodimers associate with a CD74 trimer in the ER to form a heterononamer. CD74 is sequentially degraded, leaving CLIP fragments, which are removed by HLA-DM to allow high-affinity peptide binding.
HLA-DM stabilizes MHC II molecules until antigenic peptides are bound. In B-cells, this interaction is regulated by HLA-DO. Lysosomal acidification enhances proteolysis and peptide loading efficiency.
HLA and MHC antibodies are crucial in immunopeptidomics, aiding identification and characterization of neoantigens through LC-MS/MS analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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