Thermo Fisher Scientific Ephexin-1/EPHEXIN Polyclonal Antibody
Ephexin-1/EPHEXIN 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. KLH-conjugated synthetic peptide를 면역원으로 사용. Protein A 정제, 고농도(1 mg/mL) 액상 형태.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human EPHEXIN (amino acids 451–550) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ephexin-1 acts as a guanine nucleotide exchange factor (GEF) that differentially activates GTPases RHOA, RAC1, and CDC42.
It plays a critical role in axon guidance by regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis.
Upon activation by ephrin through EPHA4, the GEF activity shifts toward RHOA, promoting cone retraction and reducing RAC1- and CDC42-mediated growth cone extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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