Thermo Fisher Scientific Human BRAF [V599E], GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

416-766; V599E

Tag

GST-tag

Molecular weight

67.1 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris, pH 7.5, with 50% glycerol, 0.02% Triton X-100, 2mM DTT, 150mM NaCl, 0.5mM EDTA

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Protein Form: Catalytic

Target Information

BRAF acts upstream of MEK in the MAP kinase pathway and is involved in cell division, differentiation, and secretion. The BRAF mutant V599E is oncogenic and found in a variety of cancers. Mutations in BRAF (V599E Mutant) have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF (V599E Mutant).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.