Thermo Fisher Scientific MAPKAPK5 Monoclonal Antibody (OTI5E1), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI5E1

Immunogen

Human recombinant protein fragment corresponding to amino acids 197-471 of human MAPKAPK5 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PRAK,uniProtId:Q8IW41-1,ncbiNodeId:9606,antigenRange:197-471,antigenLength:473,antigenImageFileName:CF804136_PRAK_Q8IW41-1_House_mouse.svg,antigenImageFileNamePDP:CF804136_PRAK_Q8IW41-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

MAPKAPK5 (PRAK) is activated in response to cellular stress and pro-inflammatory cytokines. Activity of this kinase is regulated by p38 alpha and p38 beta. The protein encoded by PRAK is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of PRAK encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.