Thermo Fisher Scientific APC15 Polyclonal Antibody
Human APC15 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 세포주기 조절 연구용으로 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human APC15. Recombinant protein control fragment (Product #RP-97313) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638066 |
Product Specific Information
Immunogen sequence:
PSLFPRVTET LWFNLDRPCV EETELQQQEQ QHQAWLQSIA EKDNNLVPIG KPASEHYDDE EEED
Highest antigen sequence identity:
Mouse – 100%, Rat – 100%
Target Information
ANAPC15 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.
Within the complex, it plays a role in the release of the mitotic checkpoint complex (MCC) from the APC/C. It is not required for APC/C activity itself but promotes the turnover of CDC20 and MCC on the APC/C, thereby contributing to the responsiveness of the spindle assembly checkpoint. It is also required for degradation of CDC20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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