Thermo Fisher Scientific PKLR Polyclonal Antibody
PKLR 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, ICC/IF에 적합합니다. 인간 및 랫트 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로 사용되며 장기 보관 시 -20°C에서 안정적입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–260 of Pyruvate Kinase (liver/RBC) (Uniprot ID: P30613) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11156033 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, rat liver, PKLR-transfected 293T
- Predicted reactivity: Mouse (92%), Rat (92%), Dog (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis.
Defects in this enzyme, due to gene mutations or genetic variations, are a common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).
Multiple transcript variants encoding different isoforms have been found for this gene.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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