Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), eFluor 450, eBioscience
마우스 GITR(TNFRSF18)를 인식하는 DTA-1 단클론 항체로, eFluor 450 형광 표지. Flow cytometry에 최적화되어 있으며, 405/445 nm에서 여기/방출. Treg 세포 분석 및 면역 조절 연구용. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DTA-1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1944395 |
Additional Available Formats
- APC
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The DTA-1 monoclonal antibody reacts with mouse GITR (Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18).
In naive mice, GITR is expressed predominantly by CD4⁺CD25⁺ T regulatory cells (Treg) and by CD25⁺ CD4⁺ CD8⁻ thymocytes.
Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ-specific autoimmune disease.
Applications Reported:
This DTA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.06 µg per test (in 100 µL volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
Ensure the instrument supports this fluorochrome.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR, is encoded by the TNFRSF18 gene located at 1p36.33.
This receptor is upregulated upon T-cell activation and plays a key role in maintaining immunological self-tolerance via CD25⁺CD4⁺ regulatory T cells.
Knockout studies in mice suggest its involvement in CD3-driven T-cell activation and apoptosis regulation.
Three alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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