Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI1A10), TrueMAB
MAP2K1(MEK1) 단백질을 인식하는 Mouse monoclonal 항체로, WB, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat, Dog 반응성. Lyophilized 형태로 제공되며, PBS buffer에 보관. 연구용으로 세포 성장 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A10 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase involved in mitogen-activated protein kinase (MAPK) cascades, regulating cell growth and differentiation.
It is activated by various growth factors, cytokines, and membrane depolarization.
Phosphorylation at serine 218 and 222 by Raf1 activates Mek1.
MAP2K1 participates in stress response, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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