Thermo Fisher Scientific V5 Tag Monoclonal Antibody (2F11F7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 14 publications 14 publications

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 7 publications 7 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2F11F7

Immunogen

sequence -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533339

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.