Thermo Fisher Scientific Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1154

Immunogen

Synthetic phospho peptide derived from a region of the human Progesterone Receptor (hPR) protein surrounding the phosphorylated Ser190 residue. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Progesterone Receptor,uniProtId:P06401-1,ncbiNodeId:9606,antigenRange:190,antigenLength:933,antigenImageFileName:37-8200_Progesterone_Receptor_P06401-1_House_mouse.svg,antigenImageFileNamePDP:37-8200_Progesterone_Receptor_P06401-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein G

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533343

Product Specific Information

37-8200 detects Phospho-Progesterone Receptor in Human samples.

37-8200 has been successfully used in western blot, immunofluorescence and immunohistochemistry in human samples. Phospho-Progesterone Receptor (Ser 190) antibody detects both progesterone receptor A and B forms when phosphorylated at Ser26 and Ser190, respectively.

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.