Thermo Fisher Scientific TDG Monoclonal Antibody (OTI3A12), TrueMAB
TDG 단백질을 인식하는 OTI3A12 클론의 단일클론 항체로, Western blot 및 IHC(P)에서 검증됨. Human, Mouse, Rat 반응성. Lyophilized 형태로 제공되며, PBS(pH 7.3, 8% trehalose) 버퍼에 보존. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 160–253 of human TDG produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The TDG protein belongs to the TDG/mug DNA glycosylase family.
Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mismatched thymine.
It also removes thymine from C/T and T/T mispairings, and can excise uracil and 5-bromouracil from mispairings with guanine.
This enzyme plays a central role in cellular defense against genetic mutations caused by spontaneous deamination of 5-methylcytosine and cytosine.
A pseudogene may exist on the p arm of chromosome 12.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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