Thermo Fisher Scientific CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), PE, eBioscience
CD68 단백질을 인식하는 단클론 항체로, 인간 유래 세포에 반응합니다. PE 형광 표지로 유세포분석에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 단핵구, 대식세포 등 골수 유래 세포의 표면 및 세포내 CD68 검출에 적합합니다.
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Thermo Fisher Scientific CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), PE, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 24 publications |
| Immunomicroscopy (IM) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioY1/82A (Y1/82A) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10804042 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 647
- Biotin
- FITC
- PE-Cyanine7
- PE-eFluor 610
- Request custom conjugation
Product Specific Information
The eBioY1/82A antibody reacts with CD68, a member of the sialomucin family closely related to lysosomal-associated membrane proteins (LAMPs) and scavenger receptors. CD68 is predominantly intracellular, located mainly in late endosomes, but also detectable on the surface of myeloid-derived cells such as monocytes, macrophages, dendritic cells, neutrophils, and basophils.
Functionally, CD68 may play a role in antigen processing or presentation and in protecting lysosomal membranes from hydrolytic enzymes. Expression has also been observed in activated T cells and B lymphocytes.
Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes, while intracellular staining detects CD68 in about 50% of B cells and most monocytes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test in a final volume of 100 µL (cell count 10⁵–10⁸ cells/test).
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein mainly expressed in lysosomes of monocytes and macrophages, and to a lesser extent in dendritic cells and granulocytes. It is involved in phagocytosis and lysosomal metabolism, as well as in cell-cell and cell-pathogen interactions.
CD68 expression has been noted in tonsillar interdigitating reticulum cells, histiocytic lymphoma, histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated CD68 levels have also been found in CD34+ cells in various malignancies, including AML.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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