Thermo Fisher Scientific Phospho-CHK2 (Thr387) Polyclonal Antibody
CHK2 단백질의 Thr387 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 액상 형태, -20°C 보관. DNA 손상 반응 및 암 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with various cancers. CHK2 is activated by DNA damage and phosphorylates several cell cycle control modulators, including tumor suppressor proteins such as BRCA1. This phosphorylation allows BRCA1 to promote cell survival after DNA damage. CHK2 is considered a putative tumor suppressor, and mutations in the CHK2 gene are linked to Li-Fraumeni syndrome as well as predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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