Thermo Fisher Scientific WTAP Polyclonal Antibody
WTAP 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. 핵 및 세포질 내 WTAP 검출에 유용합니다.
- 카탈로그번호
- PA5144681
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within human WTAP aa 1–300/396 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.4) with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3093526 |
Product Specific Information
- Positive control: K562, Jurkat, Hela, HepG2, 293T, NIH/3T3, PC-12 cell lysates, human breast carcinoma, human colon, mouse and rat small intestine tissues
- Predicted band size: 44 kDa
- Subcellular Location: Nucleus speckle, Nucleus (nucleoplasm), Cytoplasm
Target Information
Wilms’ tumor (WT) is an embryonal malignancy of the kidney affecting approximately 1 in 10,000 infants and occurs in both sporadic and inherited forms.
The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element shared with the early growth response (Egr) family of Zn²⁺ finger transcriptional activators, functioning as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may regulate mRNA splicing.
WTAP localizes to nuclear speckles and regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and reduces WT1 DNA-binding ability. Two isoforms exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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