Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (NIMP-R14)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Flow Cytometry (Flow)

1:50

-

ELISA (ELISA)

1:50

-

Functional Assay (Functional)

1:50

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

NIMP-R14

Immunogen

Purified BALB/c mouse neutrophils. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ly-6G/Ly-6C,uniProtId:P35461-1,ncbiNodeId:10090,antigenRange:1-134,antigenLength:134,antigenImageFileName:MA1-40038_Ly-6GLy-6C_P35461-1_Rat.svg,antigenImageFileNamePDP:MA1-40038_Ly-6GLy-6C_P35461-1_Rat_PDP.jpeg,sortOrder:1},{targetFamily:Ly-6G/Ly-6C,uniProtId:P0CW02-1,ncbiNodeId:10090,antigenRange:1-131,antigenLength:131,antigenImageFileName:MA1-40038_Ly-6GLy-6C_P0CW02-1_Rat.svg,antigenImageFileNamePDP:MA1-40038_Ly-6GLy-6C_P0CW02-1_Rat_PDP.jpeg,sortOrder:2},{targetFamily:Ly-6G/Ly-6C,uniProtId:P0CW03-1,ncbiNodeId:10090,antigenRange:1-131,antigenLength:131,antigenImageFileName:MA1-40038_Ly-6GLy-6C_P0CW03-1_Rat.svg,antigenImageFileNamePDP:MA1-40038_Ly-6GLy-6C_P0CW03-1_Rat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077221

Product Specific Information

This clone is highly specific for murine Ly-6G and Ly-6C. It has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with this antibody in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

The endotoxin concentration is < 24 EU/mg, determined with LAL Assay.

Suggested positive control: Mouse neutrophils; Suggested negative control: Mouse thymocytes

Target Information

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.