Thermo Fisher Scientific KAP1 (TRIM28) Monoclonal Antibody (OTI2H10)
KAP1(TRIM28) 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human 시료에 반응하며, Affinity chromatography로 정제된 1 mg/mL 액상 형태. -20°C 보관, PBS/glycerol buffer 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H10 |
| Immunogen | Full length human recombinant protein of human TRIM28 (NP_005753) produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and functions as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance repression efficiency. KAP-1 also interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin regions containing HP1α, thereby regulating transcriptional repression. Mutant KAP-1 proteins that bind KRAB but not HP1 lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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