Thermo Fisher Scientific USP28 Polyclonal Antibody
Thermo Fisher Scientific의 USP28 Polyclonal Antibody는 Human, Mouse, Rat에 반응하는 Rabbit IgG 항체입니다. Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human USP28. Recombinant protein control fragment (Product #RP-89228). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2649336 |
Product Specific Information
Immunogen sequence:
SWEEVERDSY GGLRNVSAYC LMYINDKLPY FNAEAAPTES DQMSEVEALS VELKHYIQED NWRFEQEVEE WEEEQSCKIP QMESSTNSSS QDYSTSQEPS VASSHGVRCL SSEHAVIVKE QTAQAIANTA RA
Highest antigen sequence identity to the following orthologs:
- Mouse: 83%
- Rat: 88%
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.
An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families:
- Ubiquitin C-terminal hydrolases
- Ubiquitin-specific processing proteases (USPs)
- OTU-domain ubiquitin-aldehyde-binding proteins
- Jab1/Pad1/MPN-domain-containing metallo-enzymes
Among these, USPs are the most widespread and conserved deubiquitinating enzymes across evolution. They release ubiquitin from conjugated proteins and share conserved catalytic domains (Cys and His boxes) with variable terminal extensions involved in substrate recognition, localization, and protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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