Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser217, Ser221) Recombinant Rabbit Monoclonal Antibody (8O3V3)
Phospho-MEK1/MEK2 (Ser217, Ser221) 특이적 recombinant rabbit monoclonal antibody. Western blot, ELISA, IP에 적합. Human 및 Mouse 반응성. 고순도 affinity chromatography 정제, 안정적 PBS/glycerol buffer 보관.
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Applications and Tested Dilution
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 1–4 µg antibody for 800–1,000 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8O3V3 |
| Immunogen | A synthetic phosphorylated peptide around S217 & S221 of human MEK1/MEK2 (NP_002746.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.42 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094389 |
Product Specific Information
Immunogen sequence: DSMAN
Target Information
MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In the brain, this pathway also plays a key role in synaptic function (Adams and Sweatt, 2002). Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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