Thermo Fisher Scientific Themis Recombinant Rabbit Monoclonal Antibody (JE55-73)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE55-73

Immunogen

Synthetic peptide within C-terminal Human Themis if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Themis,uniProtId:Q8N1K5-1,ncbiNodeId:9606,antigenRange:641,antigenLength:641,antigenImageFileName:MA5-42501_Themis_Q8N1K5-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42501_Themis_Q8N1K5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2911642

Product Specific Information

Positive Control: Jurkat cell lysates, 293T, rat spleen tissue, human appendix tissue, human spleen tissue, Jurkat.

Subcellular Location: Nucleus, Cytoplasm.

Target Information

Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.