Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

0.02 µg/mL

-

ELISA (ELISA)

1 µg/mL (coating), 0.06-0.3 µg/mL (detection)

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

C8.6

Immunogen

COS cell-derived recombinant human IL-12, p40 subunit

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 4% BSA

Contains

proprietary preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223555

Product Specific Information

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.

The M121B IL12 antibody (biotinylated conjugate of clone C8.6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M122 (clone 20C2). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.06 - 0.3 µg/mL for detection with 50 µl/well.

Biotinylated antibody M121B (clone C8.6) and antibody M122 (clone 20C2) have successfully been used in combination with recombinant IL12 p70 protein SIL12 in ELISA applications.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.