Thermo Fisher Scientific IL-12 p70 Monoclonal Antibody (20C2)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 18 publications 18 publications

Neutralization (Neu)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Bacteria, Human

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

20C2

Immunogen

Recombinant human IL-12 (p70)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

RRID

AB_223556

Product Specific Information

M122 targets IL-12 in ELISA, and WB applications and shows reactivity with Human samples.

The M122 immunogen is recombinant human IL-12 (p70).

M122 detects IL-12 which has a predicted molecular weight of approximately 23 kDa.

The M122 IL12 antibody (clone 20C2) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M121B (biotinylated conjugate of clone C8.6) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M122 (clone 20C2) and biotinylated antibody M121B (clone C8.6) have successfully been used in combination with recombinant IL12 p70 protein SIL12 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system`s response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.