Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience
마우스 IFN-γ를 인식하는 중화능 단클론 항체로, 기능 등급(FG) 정제형입니다. ELISA, Flow Cytometry, Neutralization 등 다양한 분석에 사용 가능하며, 낮은 엔도톡신 수준과 높은 순도를 제공합니다. 세포 활성화 및 사이토카인 연구용으로 적합합니다.
- 카탈로그번호
- 16-7311-xxx (5개 옵션)
- 판매단위
- pk
카탈로그
5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience
Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | - | 66 publications |
| ELISA | - | 4 publications |
| Neutralization | Assay-Dependent | 7 publications |
| Functional Assay | Assay-Dependent | 15 publications |
| T-Cell Activation (TCA) | - | 1 publication |
| Affinity Purification (AP) | - | 1 publication |
| In vitro Assay (IV) | - | 5 publications |
| Miscellaneous PubMed | - | 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | XMG1.2 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2866271 |
Available Formats:
- Unconjugated
- Alexa Fluor 488, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 660
- FITC, PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available upon request
Product Specific Information
The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and functions as a neutralizing antibody. Mouse IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with TNF alpha to inhibit proliferation of normal and transformed cells. Glycosylation does not affect its biological activity.
Applications Tested:
- Verified for low endotoxin levels (LAL assay)
- Tested for ELISA capture and neutralization bioassays
Neutralization Performance:
- ND50: 15 ng/mL antibody inhibits 50% of bioactivity of 1 ng/mL mouse IFN gamma in EMCV assay of L929 cell protection.
ELISA Use:
- Capture concentration: 0.25–1 µg/mL
- Standard curve range: 2000 pg/mL – 15 pg/mL
- Recommended detection antibody: Biotin R4-6A2 (13-7312)
- Standard: Recombinant mouse IFN gamma (39-8311)
Storage and Handling:
- Use in sterile environment
- 0.2 µm post-manufacturing filtered
- Purity: >90% (SDS-PAGE)
- Endotoxin: <0.001 ng/µg antibody (LAL assay)
- Aggregation: <10% (HPLC)
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits. The biological activity is species-specific; human IFN gamma does not cross-react with mouse IFN gamma.
Functions:
- Antiviral and antiproliferative activity
- Induction of MHC class I and II
- Macrophage activation
- Enhanced immunoglobulin secretion by B cells
- Cytokine regulation via JAK-STAT pathway
Regulation:
- Upregulated by IL2, FGF basic, EGF
- Downregulated by vitamin D3 or DMN
- Mutations associated with aplastic anemia
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.