Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade

Applications and Tested Dilution

Product Specifications

Available Formats:

• Unconjugated
• Alexa Fluor 488, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 660
• FITC, PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available upon request

Product Specific Information

The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and functions as a neutralizing antibody. Mouse IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with TNF alpha to inhibit proliferation of normal and transformed cells. Glycosylation does not affect its biological activity.

Applications Tested:

• Verified for low endotoxin levels (LAL assay)
• Tested for ELISA capture and neutralization bioassays

Neutralization Performance:

• ND50: 15 ng/mL antibody inhibits 50% of bioactivity of 1 ng/mL mouse IFN gamma in EMCV assay of L929 cell protection.

ELISA Use:

• Capture concentration: 0.25–1 µg/mL
• Standard curve range: 2000 pg/mL – 15 pg/mL
• Recommended detection antibody: Biotin R4-6A2 (13-7312)
• Standard: Recombinant mouse IFN gamma (39-8311)

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtered
• Purity: >90% (SDS-PAGE)
• Endotoxin: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits. The biological activity is species-specific; human IFN gamma does not cross-react with mouse IFN gamma.

Functions:

• Antiviral and antiproliferative activity
• Induction of MHC class I and II
• Macrophage activation
• Enhanced immunoglobulin secretion by B cells
• Cytokine regulation via JAK-STAT pathway

Regulation:

• Upregulated by IL2, FGF basic, EGF
• Downregulated by vitamin D3 or DMN
• Mutations associated with aplastic anemia

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.