Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 660, eBioscience

Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 570
• eFluor 615
• Request custom conjugation

Product Specific Information

This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues and distinguishes astrocytes from other glial cells. Three splice variants exist, with alpha-GFAP being predominant. GFAP can co-assemble with vimentin and nestin but does not require these associations for assembly. Mutations in GFAP cause Alexander disease and overexpression is observed in certain glial-derived tumors.

Applications Reported:
Used for intracellular staining (Flow Cytometry), immunohistochemistry (frozen and paraffin-embedded tissue), and immunocytochemistry.

Applications Tested:
Validated in IHC on formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval; optimal performance ≤10 µg/mL. Titration recommended.

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, excited by red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtered post-manufacturing (0.2 µm).

Target Information

GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed in astrocytes, satellite cells, and non-myelinating Schwann cells. It serves as a key astrocytic marker and is highly expressed in astrocyte-derived brain tumors. GFAP is also found in lens epithelium, Kupffer cells, and salivary gland tumors. Mutations in GFAP cause Alexander disease. Multiple transcript variants exist due to alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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