Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 660, eBioscience
GFAP 단백질을 인식하는 GA5 클론 단일클론 항체로, eFluor 660 형광염료가 결합되어 있습니다. 인간, 마우스, 랫 등 다양한 종에서 반응하며, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합합니다. 신경교세포 표지 및 뇌종양 연구용으로 활용됩니다.
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Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 19 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 6 publications |
| Flow Cytometry (Flow) | Assay-Dependent | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rabbit, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GA5 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574407 |
Additional Formats
Product Specific Information
This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues and distinguishes astrocytes from other glial cells. Three splice variants exist, with alpha-GFAP being predominant. GFAP can co-assemble with vimentin and nestin but does not require these associations for assembly. Mutations in GFAP cause Alexander disease and overexpression is observed in certain glial-derived tumors.
Applications Reported:
Used for intracellular staining (Flow Cytometry), immunohistochemistry (frozen and paraffin-embedded tissue), and immunocytochemistry.
Applications Tested:
Validated in IHC on formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval; optimal performance ≤10 µg/mL. Titration recommended.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, excited by red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtered post-manufacturing (0.2 µm).
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed in astrocytes, satellite cells, and non-myelinating Schwann cells. It serves as a key astrocytic marker and is highly expressed in astrocyte-derived brain tumors. GFAP is also found in lens epithelium, Kupffer cells, and salivary gland tumors. Mutations in GFAP cause Alexander disease. Multiple transcript variants exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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