Thermo Fisher Scientific PML Polyclonal Antibody
Thermo Fisher Scientific의 PML Polyclonal Antibody는 인간 PML 단백질을 인식하는 Rabbit IgG 항체입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500–1:2,000
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 375 and 425 of promyelocytic leukemia (PML) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For Research Use Only. Not for use in diagnostic procedures.
- Not for resale without express authorization.
Target Information
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, involved in nucleotide excision repair (NER). It interacts with 3-methyladenine-DNA glycosylase (MPG), suggesting a role in DNA damage recognition in base excision repair.
This protein contains an N-terminal ubiquitin-like domain that interacts with the 26S proteasome and ubiquitin protein ligase E6AP, suggesting involvement in ubiquitin-mediated proteolytic pathways.
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