Thermo Fisher Scientific LIF Polyclonal Antibody
LIF 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC, ELISA 등에 사용 가능. Human, Mouse, Rat 반응성. Protein A로 정제된 1 mg/mL 액상 제품으로, -20°C에서 보관. 줄기세포 분화 억제 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human LIF (amino acids 101–202) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Leukemia inhibitory factor (LIF) is a 20 kDa protein belonging to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a LIF-specific subunit (gp190 or LIFR) and gp130, which is shared among IL-6 family members. LIF expression occurs in thymus, lung, and neuronal tissues, and can be upregulated by pro-inflammatory cytokines such as TNFα and IL-17. Elevated LIF levels are associated with rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF is known for inhibiting embryonic stem cell differentiation in mice and maintaining stem cell self-renewal. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity, though stem cell differentiation inhibition is mouse-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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