Thermo Fisher Scientific Caspase 4 Monoclonal Antibody (17D9)

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

17D9

Immunogen

Recombinant p30 subunit of mouse caspase 11.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.4 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_779521

Product Specific Information

Recommended positive controls: EL4.

MA1-24703 is expected to cross react with rat due to sequence homology.

Target Information

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.