Thermo Fisher Scientific CD8A (Cytotoxic- and Suppressor T-Cell Marker) Polyclonal Antibody
CD8A 단백질을 표적하는 토끼 폴리클로날 항체로, 인간 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능하며, 단백질 A로 정제된 액상 형태입니다. 보존제 없이 PBS(pH 7.4)에 보관하며, -20°C에서 냉동 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length human CD8A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family that plays an essential role in T cell signal transduction, differentiation, and activation. CD8 is mainly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules. In contrast, CD4 interacts with MHC class II molecules. CD8α is critical for MHC-I binding and is also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers. Engagement of MHC-I/peptide complexes on antigen-presenting cells recruits Lck kinase, initiating lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are key in eliminating pathogens and tumor cells. Differentiation of naive CD8⁺ T cells into CTLs is promoted by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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