Thermo Fisher Scientific CD8A (Cytotoxic- and Suppressor T-Cell Marker) Polyclonal Antibody
Human CD8A 단백질을 인식하는 Rabbit Polyclonal Antibody로, 세포독성 및 억제성 T세포 마커 검출에 사용됩니다. IHC(P), IHC(PFA), Flow Cytometry에 적합하며, Protein A/G로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD8A (Cytotoxic- and Suppressor T-Cell Marker) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment from human CD8 C-terminal cytoplasmic domain of alpha chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction and T cell differentiation and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with T cell receptor (TCR) for MHC class I molecules.
CD8α chain is essential for binding to MHC-I and is also expressed on subsets of T cells, NK cells, monocytes, and dendritic cells as homodimers.
Ligation of MHC-I/peptide complexes triggers recruitment of Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte activation.
Activated CTLs play a crucial role in pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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