Thermo Fisher Scientific Rabbit anti-Human IgM (Heavy chain) Secondary Antibody, HRP
HRP 결합형 Rabbit anti-Human IgM (Heavy chain) 2차 항체로, Western blot, IHC, ELISA 등에 사용 가능. 고감도·특이성 검증 완료. Lyophilized 형태로 안정적이며, 장기보관 시 -20°C에서 보관 권장.
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Thermo Fisher Scientific Rabbit anti-Human IgM (Heavy chain) Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2535684 |
| Target | IgM (Heavy chain) |
| Antibody Form | Whole Antibody |
Available Formats
- Biotin conjugate
- FITC conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
- Each lot’s sensitivity is confirmed using ELISA, and specificity is validated by immunoelectrophoresis (IEP).
- Rehydrate with 1.1 mL of deionized water and let stand for 30 minutes at room temperature to dissolve. Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum immunoglobulins or light chains of human immunoglobulins.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing a host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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