
Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP
HRP 결합형 Rabbit anti-Rat IgG (H+L) 2차 항체로, Western blot, IHC, ELISA 등에 사용 가능. Rat IgG에 높은 특이성과 감도를 가지며, 친화 크로마토그래피로 정제됨. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution Range |
|---|---|
| Western Blot (WB) | 1:2,000 - 1:20,000 |
| Immunohistochemistry (IHC) | 1:500 - 1:20,000 |
| ELISA | 1:500 - 1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535690 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Conjugate Formats
- AP
- Biotin
- FITC
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
- Each antibody lot’s sensitivity is confirmed using ELISA.
- Specificity is verified by immunoelectrophoresis (IEP).
- To reconstitute, add 1.1 mL of deionized water and let stand 30 minutes at room temperature.
- Centrifuge to remove particulates and prepare fresh working dilutions daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins.
Target Information
Anti-Rat secondary antibodies are affinity-purified and show high specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enable use of multiple detection systems (HRP, AP, fluorescence) and increase sensitivity through signal amplification.
Typically generated by immunizing the host with pooled immunoglobulins from the target species, they can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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