Thermo Fisher Scientific Phospho-Cyclin B1 (Ser133) Polyclonal Antibody
Cyclin B1의 Ser133 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, WB, ICC/IF, Flow, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A 정제 및 안정적 액상 형태로 제공. 세포주기 조절 및 암 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-Cyclin B1 (Ser133) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein essential for control of the cell cycle at the G2/M transition. It forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutive and one cell cycle-regulated, expressed predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and undergoes APC-dependent destruction at the end of mitosis, which is critical for cell cycle progression.
Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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