Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (C80, 3544)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Antibody is tested in direct ELISA.
• For immunohistochemistry (PFA-fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of the T cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on the surface of activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes.
The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes by binding to CD28 and CTLA4 ligands.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.