Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (C80, 3544)
인체 CD80 단백질을 인식하는 mouse monoclonal antibody로, IHC(P) 및 Peptide Array에 사용 가능. Protein A/G로 정제된 액상 항체로 4°C 보관. T세포 활성화 연구 및 면역조직화학 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C80, 3544 |
| Immunogen | Recombinant fragment of human CD80 protein (around aa 35–142) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris Buffer with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of the T cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes through binding to CD28 and CTLA4. CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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