Thermo Fisher Scientific LentiArray Human GPCR CRISPR Library

Invitrogen™ LentiArray™ Human GPCR CRISPR Library

The Invitrogen™ LentiArray™ Human GPCR Library targets 446 genes with up to 4 gRNAs per gene target (pooled in a single well), for a total of 1,784 gRNAs.
Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1×10⁸ TU/mL (functional titer determined by antibiotic resistance).

Overview

G protein-coupled receptors (GPCRs) regulate a wide range of physiological and disease processes. Nearly 30% of FDA-approved drugs target GPCRs, making them one of the most druggable gene classes.
The gene targets in this library were selected using up-to-date genome databases, including NCBI RefSeq, Gene Ontology Consortium (GO), and HUGO Gene Nomenclature Committee (HGNC).

Built to Bring You Success

LentiArray CRISPR libraries use Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm, incorporating the latest research and in-house experience to maximize editing efficiency and specificity.
Each gene target includes up to four high-quality gRNAs designed to knock out all known isoforms, ensuring efficient knockout across diverse cell types.

Flexible Experimental Design

The library employs a two-vector system, expressing Cas9 nuclease and gRNA on separate lentiviral constructs.
This allows users to control when and how genome-editing tools are delivered. The LentiArray Cas9 lentivirus expresses a human codon-optimized Cas9 with a blasticidin-resistance gene under the EF-1α (EFS) promoter.

Users can:

• Co-infect cells with both Cas9 and gRNA lentiviruses for immediate editing
• Establish stable Cas9-expressing cell lines using only the Cas9 lentivirus

The gRNA lentiviruses do not contain fluorescent markers, allowing the use of reporters of any wavelength and enabling multiplexed experiments.
For challenging cell lines, puromycin resistance allows enrichment of edited cells and stronger phenotypes.

Learn more about LentiArray CRISPR libraries ›

Optimize Your Assay Design

High-quality controls are essential for successful screening.
The LentiArray CRISPR libraries include a suite of controls to support assay development and provide confidence in hit identification.

Learn more about LentiArray controls ›

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