Thermo Fisher Scientific RBM8A Monoclonal Antibody (4C4)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunocytochemistry (ICC/IF)

1:15

-

Flow Cytometry (Flow)

1 µg/1x10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Xenopus

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4C4

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896810

Product Specific Information

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Western blot detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target Information

This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.