Thermo Fisher Scientific PNMT Monoclonal Antibody (OTI1B11), TrueMAB
PNMT 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. HEK293T 세포에서 발현된 full-length human PNMT를 면역원으로 사용. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B11 |
| Immunogen | Full length human recombinant protein of human PNMT produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The PNMT gene product catalyzes the final step in catecholamine biosynthesis, converting norepinephrine to epinephrine (adrenaline).
This enzyme also exhibits beta-carboline 2N-methyltransferase activity and plays a crucial role in regulating epinephrine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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