Thermo Fisher Scientific p53 Monoclonal Antibody (OTI1D8), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest.

p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and acts as a tumor suppressor. Mutations in the TP53 gene are common in various human cancers and can lead to loss of tumor suppressor function. Germline mutations are also linked to hereditary cancer syndromes such as Li-Fraumeni syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.