Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405

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인간 IgG(H+L)에 특이적인 염소 유래 교차흡착 2차 항체로, Alexa Fluor Plus 405 형광 표지 제공. 면역세포염색(ICC/IF)에 적합하며, 높은 특이성과 낮은 비특이적 결합으로 선명한 형광 이미징 가능. 실험 간 교차반응 최소화.

카탈로그번호: A48275
브랜드: Thermo Fisher Scientific
카테고리: Polyclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific A48275 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 1 mg pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405

Applications and Tested Dilution

Immunocytochemistry (ICC/IF): 1–10 µg/mL

Product Specifications

항목	내용
Species Reactivity	Human
Host / Isotype	Goat / IgG
Class	Polyclonal
Type	Secondary Antibody
Immunogen	Gamma Immunoglobulins Heavy and Light chains
Conjugate	Alexa Fluor™ Plus 405
Excitation / Emission Max	404 / 455 nm
Form	Liquid
Concentration	2 mg/mL
Purification	Affinity chromatography
Storage buffer	Proprietary buffer, pH 6.5
Contains	0.016% Methylisothiazolone, 0.016% Bromonitrodioxane
Storage conditions	4°C, store in dark
Shipping conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2890321
Target	IgG
Cross Adsorption	Against serum proteins from bovine, mouse, and rabbit
Antibody Form	Whole Antibody

Product Specific Information

To minimize cross-reactivity, the goat anti-human IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, mouse, and rabbit. Cross-adsorption is a purification step that increases antibody specificity, reducing background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species, allowing highly specific antibodies to pass through.

Usage Notes:

Centrifuge the conjugate solution briefly before use and use only the supernatant to avoid aggregates.
Optimize antibody dilution empirically for each application.

Specificity:
This antibody binds to heavy chains on human IgG and light chains on all human immunoglobulins. It does not bind non-immunoglobulin human serum proteins or serum proteins/IgG from bovine, mouse, and rabbit.

Target Information

Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are used for detection, sorting, or purification of target antibodies. These reagents enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification through multiple binding events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.